cellometer cell counter Search Results


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ivis spectrum system  (Revvity)
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THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Ivis Spectrum System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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opera phenix high content screening system  (Revvity)
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Revvity opera phenix high content screening system
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
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chemdraw professional 20 0 program  (Revvity)
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Revvity chemdraw professional 20 0 program
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
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phenovue cell painting jump kit  (Revvity)
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Revvity phenovue cell painting jump kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Phenovue Cell Painting Jump Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flexdrop iv dispenser  (Revvity)
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THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
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filtermats  (Revvity)
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THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
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THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
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Image Search Results


THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system (IVIS) images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.

Journal: Frontiers in Immunology

Article Title: Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway

doi: 10.3389/fimmu.2025.1577234

Figure Lengend Snippet: THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system (IVIS) images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.

Article Snippet: Fourteen days post-surgery, in vivo 3D optical imaging was performed using the IVIS Spectrum system (PerkinElmer, USA) to assess transfection efficiency.

Techniques: In Vivo Imaging, Real-time Polymerase Chain Reaction, Immunofluorescence, Micro-CT, Staining, Immunohistochemical staining